Skip to main content

Table 1 PCR primers for cDNA cloning and construction of expression vector.

From: Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappaL.)

Primer name

DNA sequence (5' to 3')

Dp-A

ACIGSITWYCAYTTYCARCC*

Dp-C

YTCYTCITYNGGCCAYTG*

Gene Racer 5'primer

CGACTGGAGCACGAGGACACTGA

5Ra-EHa

CACCAGGTCGTATGATACGGCATGCCCCC

Gene Racer 3'primer

GCTGTCAACGATACGCTACGTAACG

3Ra-EHa

GCGGCAGTGTGATGCACGTGATGAAGGCTG

EHa-FwA

ACTCTCGTCACCCAAAG

EHa-RvI

AGAGCTATCAAACGAACTCT

EHa-EcoRI.Q34

AGGAATTCAGCGGTTCCAACAACCGTA**

EHa-XbaI

GTTCTAGAGCTGCACTTTTTACGGTCTG**

  1. *N = (A, T, G, C), R = (A, C), S = (C, G), Y = (C, T), W = (A, T), I = inosine
  2. **Bold characters indicate the sequence of restriction sites.