Characterization of recombinant β-fructofuranosidase from Bifidobacterium adolescentisG1

BMC Chemistry

Table 2 Effects of various metal salts or chemical reagents on recombinant β-FFase.

Reagent	Relative activity (%)
None	100
AgNO₃	0
CuSO₄	51.8
MgCl₂	99.1
FeCl₃	110.6
CoCl₂	105.1
MnCl₂	100.2
SnCl₂	99.2
ZnCl₂	121.1
CaCl₂	102.1
HgCl₂	0
SDS	0
Deoxycholic acid sodium salt	136.9
TritonX-100	137.8
Tween 80	137.7

A reaction mixture containing 25 μl of enzyme solution, 25 μl of 0.2 M sodium phosphate buffer (pH 5.7), 10 μl of each chemical (10 mM), 15 μl of Milli-Q water and 25 μl of 40 mM 1-kestose was incubated at 37°C for 10 min.

ISSN: 2661-801X