Table 1 Summary of purification procedure recombinant β-FFase.
From: Characterization of recombinant β-fructofuranosidase from Bifidobacterium adolescentisG1
Procedure | Total protein (mg) | Total activity (units) | Specific activity (units/mg) | Purification (-fold) | Recovery (%) |
|---|---|---|---|---|---|
Crude extract | 163 | 1,480 | 9.08 | 1.00 | 100 |
Metal affinity resins | 30.0 | 600 | 20.0 | 2.20 | 40.5 |
DEAE-Sepharose CL-6B | 11.3 | 900 | 79.7 | 8.78 | 60.8 |
Toyopearl HW-55S | 3.65 | 314 | 86.0 | 9.47 | 21.2 |