Figure 4
From: Assembling proteomics data as a prerequisite for the analysis of large scale experiments
Post-processing: Clustering of polymer contaminants. A cluster dendrogram comprising 873 MS/MS mass spectra is shown. The data were recorded using a comparative ICAT/LC/ESI-MS study of proteasome subtypes from rat liver. The framed part of the dendrogram shows a cluster of 125 similar mass spectra originated by polymer contaminations due to avidin column purification steps. Half of these mass spectra falsely fitted to proteasome peptides using Mascot search algorithm. These junk mass spectra were eliminated before data were stored into SQL*LIMS™.