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Table 2 The effects of trehalose concentration on the activity (initial rates) of the α-chymotrypsin precipitated by n-propanol in octane. The experiments were carried out in triplicate. The percentage error in a set was within 7 percent.

From: Optimising biocatalyst design for obtaining high transesterification activity by α-chymotrypsin in non-aqueous media

Starting trehalose (percent wv-1) percent trehalose precipitated Enzyme loadinga (weight percentage) Initial rateb (nmoles mg-1min-1) Visual morphology of enzyme precipitate Precipitation of trehalose in propanol (control)
0 0 - 16.6 Powder (EPRP) No precipitation
2 65 38.9 40.0 Powder (EPRP) No precipitation
5 90 15.5 45.2 Powder (EPRP) No precipitation
10 94 7.5 63.2 Powder-crystalline No precipitation
15 88 5.9 120.0 Crystalline Precipitation
20 85 4.6 125.1 Crystalline Precipitation
25 78 4.0 143.2 Crystalline Precipitation
30 76 3.5 150.5 Crystalline Precipitation
35 72 3.1 152.0 Crystalline Precipitation
40 70 2.8 151.2 Crystalline Precipitation
45 65 2.3 96.4 Crystalline Precipitation
Saturated trehalose solution 60 1.5 87.4 Crystalline Precipitation
PCMC (K2SO4) - 5.8 132.5 Crystalline Precipitation
  1. a percent enzyme (w/w) = mg protein precipitated (mg of protein precipitated  +  mg of core material precipitated) × 100 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=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@B8A5@
  2. The amount of protein precipitated in each case was 0.83 mg.
  3. bInitial rates were calculated from the aliquots taken over 10 to 60 min. The percentage conversions during these time periods were 1–30 percent and in a linear range.