Figure 1

Precipitation of trehalose in the presence of protein. A solution of α-chymotrypsin (1 mg in 100 μl of 10 mM potassium phosphate buffer, pH 7.8) containing different concentrations of trehalose, was precipitated by 3 ml of n-propanol at 4°C with constant shaking at 150 rpm. The amount of precipitated trehalose was determined by HPLC as described in the experimental section. The experiments were carried out in triplicate. The error bar represents the variation in the readings.