Figure 5

Cell culture supernatant proteins of Mycobacterium tuberculosis separated by a narrow range pH gradient between pI 4 and 5 in the first dimension and SDS-PAGE in the second dimension. Spots 1 to 8 were identified as different protein species of ESAT6 with 100% sequence coverage by MALDI-MS [35]. All spots of group A have an acetylated N-terminus. Spot 4 and 8 have a truncated C-terminus.