Table 2 Immobilization of Candida rugosa lipase on MWNTs in presence of BSA. CRL (3 units) were immobilized on 1 mg of MWNTs in presence of varying amount of BSA. The hydrolytic activity was determined on MWNTs, supernatant and washings. All experiments were carried out in duplicate and the results within each pair differed by < 5%.
BSA amount | Supernatant + washings | Expressed activity (B) | Theoretical activity (A) | B/A |
|---|---|---|---|---|
0 | 0.29 | 1.39 | 2.60 | 0.53 |
0.25 | 0.61 | 1.55 | 2.39 | 0.64 |
0.5 | 1.07 | 1.52 | 1.93 | 0.78 |
1 | 1.53 | 1.35 | 1.47 | 0.88 |
2 | 1.66 | 1.31 | 1.34 | 0.97 |
3 | 2.22 | 0.54 | 0.78 | 0.69 |