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Table 1 Comparison of kinetic parameters of pectinase, xylanase and cellulase in CLEA

From: A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities

Enzyme Parameter Free enzyme CLEAs
Pectinase Vmax (mgmin-1) 170 207
  Km (mgml-1) 16 15
  Vmax/Km (min-1) 11 14
Xylanase Vmax (mgmin-1) 300 400
  Km (mgml-1) 4 5
  Vmax/Km (min-1) 75 80
Cellulase Vmax (mgmin-1) 450 500
  Km (mgml-1) 28 27
  Vmax/Km (min-1) 16 19
  1. The kinetic parameters were calculated by varying the substrate concentration. The data were fitted in Hanes-Woolf plot using Leonora software [26] as mentioned in the experimental section. The experiments were carried out in triplicate and the percentage error in each set of readings was within 3%.