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Table 1 Comparison of kinetic parameters of pectinase, xylanase and cellulase in CLEA

From: A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities

Enzyme

Parameter

Free enzyme

CLEAs

Pectinase

Vmax (mgmin-1)

170

207

 

Km (mgml-1)

16

15

 

Vmax/Km (min-1)

11

14

Xylanase

Vmax (mgmin-1)

300

400

 

Km (mgml-1)

4

5

 

Vmax/Km (min-1)

75

80

Cellulase

Vmax (mgmin-1)

450

500

 

Km (mgml-1)

28

27

 

Vmax/Km (min-1)

16

19

  1. The kinetic parameters were calculated by varying the substrate concentration. The data were fitted in Hanes-Woolf plot using Leonora software [26] as mentioned in the experimental section. The experiments were carried out in triplicate and the percentage error in each set of readings was within 3%.