Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in tetracycline hydrochloride active pharmaceutical ingredient (API) and its dosage forms

Table 9 Assay of TC-HCl API and dosage forms with HPTLC and HPLC method

No. of assay	Content (%) of Tetracycline HCl in commercial form and API
No. of assay	TTC HCl API	Brassica eye ointment	Galentic eye ointment	TTC HCl USP	Galentic skin ointment	Aurocycline skin ointment
For HPTLC
1	110.07	105.64	94.95	92.60	102.80	92.37
2	109.81	104.07	97.03	92.43	104.54	92.81
3	105.11	103.03	97.81	89.73	103.89	93.90
Average amount(%)	108.33	104.25	96.60	91.59	103.74	93.03
SD	2.28	1.31	1.48	1.61	0.88	0.78
%RSD	2.10	1.26	1.53	1.76	0.85	0.84
Mean ± SD	108.3 ± 2.28	104.5 ± 1.31	96.6 ± 1.48	91.59 ± 1.61	103.74 ± 0.88	93.03 ± 0.84
For HPLC
1	98.58	102.46	96.30	90.92	100.42	92.47
2	98.61	102.42	96.31	91.32	100.26	92.45
3	98.78	103.68	96.24	90.23	100.62	90.01
Average amount (%)	98.66	102.85	96.28	90.83	100.43	91.64
SD	0.11	0.0.72	0.04	0.55	0.18	1.42
%RSD	0.11	0.70	0.04	0.60	0.18	1.54
Mean ± %RSD	98.66 ± 0.11	102.85 ± 0.72	96.28 ± 0.04	90.83 ± 0.55	100.43 ± 0.18	91.64 ± 1.42

ISSN: 2661-801X