Table 6 Statistical parameters of ANOVA for the HPTLC method at 300 ng/band
S.No. | Parameters | Mobile phase composition | Different factors effect | ||
|---|---|---|---|---|---|
Peak area | Rf | Peak area | Rf | ||
1. | Model p-value | 0.0747 | 0.1984 | 0.2019 | 0.1489 |
2. | Lack of fit | 0.9594 | 0.8720 | 0.9627 | 0.891 |
3. | C.V. % | 1.65 | 5.59 | 3.88 | 8.01 |
4. | R-squared | 0.7506 | 0.8130 | 0.4210 | 0.7632 |
5. | Adj R-squared | 0.5428 | 0.4857 | 0.2039 | 0.4789 |
6. | Pred R-squared | 0.4968 | 0.4140 | 0.1069 | 0.4128 |
7. | Adeq precision | 6.5380 | 6.0426 | 4.8219 | 6.1978 |
8. | Equation | Peak area =   + 0.0054 − 0.0000A − 0.0000B  + 0.0000C − 0.0001AB − 0.0001AC | Rf =  0.2898 − 0.015 A − 0.0105B  + 0.0067C  + 0.0077D  + 0.005AB − 0.0077AC − 0.0067AD | Peak area =   + 0.0051  + 0.0001B’ − 0.0001A’C’  + 0.0001A’D’ | Rf =   + 0.29 − 0.0075A’ − 0.042B’ − 0.005D’ − 0.0167A’B’ − 0.0075A’C’ − 0.015A’D’ |