Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in tetracycline hydrochloride active pharmaceutical ingredient (API) and its dosage forms

Table 5 Repeatability, intermediate precision and system precision test of tetracycline -HCl at 3 concentration (n = 3)

ng/band	HPTLC method
	Intra-day precision			Intermediate precision
	MPA	SD	%RSD	MPA	SD	%RSD
160	0.0024	2.8 × 10^–5	1.2	0.0024	2 × 10^–5	0.9
200	0.0035	1.7 × 10^–5	0.5	0.0034	7 × 10^–5	2.06
240	0.0043	4.5 × 10^–5	1.0	0.0043	3 × 10^–5	0.6
System precision test with 6 repeatation at 200 ng/band

Peak area for sample application				PASS-peak area for sample scanning
200	0.00276	4.3 × 10^–5	1.56	0.00275	1.8 × 10^–5	0.65

N.B.: MPA-mean peak area (n = 3), SD-standard deviation, RSD-relative standard deviation

ISSN: 2661-801X